英文名稱    Phospho-CDK2 (Thr160)    
中文名稱    磷酸化周期素依賴激酶2抗體    
別    名    p-CDH2 (Thr160); CDH2 (phospho-Thr160); CDH2 (phospho-T160); Cdc2 related protein kinase; cdc2-related protein kinase; Cdk 2; CDK2; CDK2_HUMAN; Cell devision kinase 2; Cell division kinase 2; Cell division protein kinase 2; Cyclin dependent kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; p33 protein kinase; p33(CDK2).      
產(chǎn)品類型：磷酸化抗體     
研究領(lǐng)域    腫瘤  細(xì)胞生物  免疫學(xué)  信號轉(zhuǎn)導(dǎo)  細(xì)胞周期蛋白  轉(zhuǎn)錄調(diào)節(jié)因子  激酶和磷酸酶      
抗體來源    Rabbit    
克隆類型    Polyclonal    
交叉反應(yīng)    Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Guinea Pig,     
產(chǎn)品應(yīng)用    WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    
分 子 量    33kDa    
細(xì)胞定位    細(xì)胞核 細(xì)胞漿     
性    狀    Lyophilized or Liquid    
濃    度    1mg/ml    
免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human CDK2 around the phosphorylation site of Thr160:TY(p-T)HE     
亞    型    IgG    
純化方法    affinity purified by Protein A    
儲 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    
保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    
PubMed    PubMed    
產(chǎn)品介紹    The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq, Jul 2008].     
Cdk2為周期素依賴激酶2(Cyclin-Dependent Kinase 2)，主要參與細(xì)胞周期的調(diào)控，在細(xì)胞分化、有絲分裂中起重要作用，目前主要用于各種腫瘤的研究。


相關(guān)抗體反應(yīng)規(guī)律：
（1）初次反應(yīng)產(chǎn)生抗體：當(dāng)抗原*次進(jìn)入機(jī)體時(shí)，需經(jīng)一定的潛伏期才能產(chǎn)生抗體，且抗體產(chǎn)生的量也不多，在體內(nèi)維持的時(shí)間也較短。   
（2）再次反應(yīng)產(chǎn)生抗體：當(dāng)相同抗原第二次進(jìn)入機(jī)體后，開始時(shí)，由于原有抗體中的一部分與再次進(jìn)入的抗原結(jié)合，可使原有抗體量略為降低。隨后，抗體效價(jià)迅速大量增加，可比初次反應(yīng)產(chǎn)生的多幾倍到幾十倍，在體內(nèi)留存的時(shí)間亦較長。   
（3）回憶反應(yīng)產(chǎn)生抗體：由抗原刺激機(jī)體產(chǎn)生的抗體，經(jīng)過一定時(shí)間后可逐漸消失。此時(shí)若再次接觸抗原，可使已消失的抗體快速上升。如再次刺激機(jī)體的抗原與初次相同，則稱為特異性回憶反應(yīng)；若與初次反應(yīng)不同，則稱為非特異性回憶反應(yīng)。非特異性回憶反應(yīng)引起的抗體的上升是暫時(shí)性的，短時(shí)間內(nèi)即很快下降。


多克隆抗體的制備一般包括以下幾個(gè)步驟：
1、制備抗原。  
2、選擇實(shí)驗(yàn)動(dòng)物。
3、動(dòng)物免疫。  
4、試取血進(jìn)行測試，看看是否成功免疫。
5、如果成功免疫，殺死實(shí)驗(yàn)動(dòng)物，采集全部血清。
6、純化出抗體。  
7、鑒定抗體。包括純度以及特異性。