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34096 超靈敏發光底物

北京優尼康生物科技有限公司
會員指數: 企業認證:       

價格:電議

所在地:北京

型號:34096

更新時間:2023-08-11

瀏覽次數:882

公司地址:北京市順義區府前街56號院1號樓4層1-416?

趙先生(先生) 銷售業務員 

產品簡介

SuperSignal? WEST FEMTO超靈敏發光底物

公司簡介

北京優尼康生物科技有限公司主要經營進口、國產生物科研儀器、試劑及耗材,提供技術服務的生物公司之一。現經營的品牌及相關產品如下:

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產品說明

SuperSignal? WEST FEMTO超靈敏發光底物
SuperSignal? WEST FEMTO超靈敏發光底物
SuperSignal? WEST FEMTO超靈敏發光底物

官網:https:///order/catalog/product/34096?SID=srch-hj-34096
Description Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low-femtogram-level detection by Western blot analysis with horseradish peroxidase (HRP) enzyme.
Features of SuperSignal West Femto Maximum Sensitivity Substrate:
? ECL —enhanced chemiluminescent substrate for horseradish peroxidase (HRP)
? Sensitive—detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes when probed with appropriate primary and secondary antibodies
? Quantitative—produces quantitative signal that is measurable over two orders of magnitude
? Intense signal—easy to capture an image by exposure to film or imaging system
? Excellent signal duration—8 hours of usable light-output when conditions are optimized
? Stable reagent—kit components are stable for 1 year at 4°C or 6 months at room temperature
? Economical—optimized for extremely dilute antibody concentrations:
? 1ng to 0.2 μg/mL primary antibody (1:5000 to 1:100,000 dilution from 1 mg/mL stock)
? 2 to 10 ng/mL secondary antibody (1:100,000 to 1:500,000 dilution from 1 mg/mL stock)
When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Purified IκB was serially diluted from 100 to 1fg and then electrophoresed on a 4-20% mini gel. The protein was transferred to PVDF membrane and blocked withStartingBlock Blocking Buffer for 1 ho

Purified IκB was serially diluted from 100 to 1fg and then electrophoresed on a 4-20% mini gel. The protein was transferred to PVDF membrane and blocked withStartingBlock Blocking Buffer for 1 ho

A431cell lysate was diluted in electrophoresis reducing sample buffer at 5, 2.5, 1.25, 0.625, 0.3125, and 0.15625μg/well with a 10μL/well load. After electrophoresis, proteins were transfe

A431cell lysate was diluted in electrophoresis reducing sample buffer at 5, 2.5, 1.25, 0.625, 0.3125, and 0.15625μg/well with a 10μL/well load. After electrophoresis, proteins were transfe

NIH3T3 lysate was diluted in electrophoresis reducing sample buffer. Lane 1 co<ems></ems>ntained 5μg of NIH3T3 lysate. Five 1:1 serial dilutions were then prepared and applied at 10μL/well. After electr

NIH3T3 lysate was diluted in electrophoresis reducing sample buffer. Lane 1 contained 5μg of NIH3T3 lysate. Five 1:1 serial dilutions were then prepared and applied at 10μL/well. After electr


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